A high-throughput diagnostic method for detecting pathogenic microbes.
- Author:
Hai-hong ZHU
1
;
Han-liang JIANG
;
Zhi CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Communicable Diseases; microbiology; virology; Enterovirus; isolation & purification; Humans; Microbiological Techniques; Mumps virus; isolation & purification; Oligonucleotide Array Sequence Analysis; methods; Pseudomonas aeruginosa; isolation & purification
- From: Journal of Zhejiang University. Medical sciences 2007;36(6):524-530
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a high-throughput diagnostic method with suspension array technique for detecting pathogenic microbes.
METHODSThe probes and positive controls of 56 kinds of pathogenic microbes were designed, synthesized, and used to detect pathogenic microbes with suspension array technique.
RESULTSFluorescence signals of 56 positive controls were higher than those of the negative controls, and there was no cross-reaction between the probes and positive controls of different microbes.
CONCLUSIONBased on suspension array technique, the high-throughput diagnostic method may be useful in clinical detection of pathogenic microbes.
