Dendritic cells pulsed with glioma RNA induce immunity against intracranial gliomas.
- Author:
Jian-bo YU
1
;
Zhi-xian FENG
;
Ren-ya ZHAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Neoplasms; immunology; therapy; Cancer Vaccines; immunology; Dendritic Cells; immunology; transplantation; Glioblastoma; immunology; therapy; Immunotherapy; methods; Mice; RNA, Neoplasm; immunology; Random Allocation; T-Lymphocytes, Cytotoxic; immunology
- From: Journal of Zhejiang University. Medical sciences 2007;36(6):592-597
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the anti-tumor effect of dendritic cells (DC) pulsed with G422 glioblastomas RNA in mice bearing intracranially G422 glioblastomas.
METHODSDCs were pulsed in vitro with glioblastomas G422 cell RNA. The tumor-bearing mice were injected intratumorally or subcutaneously with pulsed DCs, PBS, non-pulsed DCs. The survival duration of mice was recorded. Serum levels of cytokine IFN-gamma, IL-2, IL-10, IL-4 were detected. Pathological examination was performed.
RESULTSThe survival duration of mice with DC-based vaccine increased significantly(P<0.01). The serum IFN-gamma level was increased (P<0.01) and IL-10 level was decreased (P<0.05) after treatment. Pathological examination showed necrotic tumor in the treatment mice.
CONCLUSIONDC vaccination can significantly increase survival duration of mice with intratumoral or subcutaneous administration of vaccines.
