Preliminary study on role of lipid rafts in receptor clustering induced by 50 Hz magnetic fields and its mechanism.
- Author:
Yong WANG
1
;
Qing CHEN
;
De-qiang LU
;
Zheng-ping XU
;
Huai JIANG
;
Wen-jun SUN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Membrane; radiation effects; Cells, Cultured; Electromagnetic Fields; Epidermal Growth Factor; metabolism; Humans; Membrane Microdomains; radiation effects; Receptor, Epidermal Growth Factor; metabolism; radiation effects; Signal Transduction; physiology; radiation effects; Sphingomyelin Phosphodiesterase; metabolism
- From: Journal of Zhejiang University. Medical sciences 2008;37(1):4-8
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship among a 50 Hz magnetic field (MF)-induced epidermal growth factor receptor (EGFR) clustering,lipid rafts and acid sphingomyelinase (ASM), and to explore its possible mechanism.
METHODSHuman amnion FL cells were exposed to 50 Hz, 0.4 mT MF for 15 min. EGF treatment was used as positive control. Nystatin was employed to study lipid rafts since it could disrupt lipid rafts structure.The EGF receptors, ASM and lipid rafts were labeled with polyclonal anti-EGFR antibody, anti-ASM antibody and FITC-Cholera toxin B, respectively. The images were observed by laser confocal scanning microscope.
RESULTBoth EGF treatment and 50 Hz MF exposure could induce EGFR clustering; however, nystatin pretreatment disrupted this effect. MF exposure turned ASM (labeled with Cy3) from a diffused state in the sham exposure group to a concentrated state on the cell membrane, which co-localized with lipid rafts (labeled with FITC).
CONCLUSIONThe results suggest that the EGFR clustering induced by 50 Hz MF depends on intact lipid rafts on cellular membrane, and the ASM might participate in the process of EGFR clustering.
