Effects of 50 Hz magnetic fields on gene expression in MCF-7 cells.
- Author:
Guang-di CHEN
1
;
De-qiang LU
;
Huai JIANG
;
Zhen-ping XU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; DNA Breaks, Double-Stranded; radiation effects; Electromagnetic Fields; Gene Expression; radiation effects; Gene Expression Profiling; Humans; Polymerase Chain Reaction; Radio Waves; Reverse Transcriptase Polymerase Chain Reaction; Tumor Cells, Cultured
- From: Journal of Zhejiang University. Medical sciences 2008;37(1):15-22
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether 50 Hz magnetic fields (MF) can change the gene expression profile in MCF-7 cells and to screen MF responsive genes.
METHODSIn vitro cultured MCF-7 cells were continuously exposed or sham-exposed to 0.4 mT of 50 Hz MF for 24 hours. Affymetrix Human Genome Genechips (U133A) were applied to analyze gene expression profiles in MF exposed and sham-exposed MCF-7 cells and the data were processed with Genechip data analysis software MAS 5.0 and DMT 3.0. Real-time RT-PCR assay was employed to examine the differentially expressed genes.
RESULTThirty differentially expressed genes were screened with 100 % consistency change calls in the MF exposed MCF-7 cells. Six independent real-time RT-PCR analyses showed that SCNN1A, METTL3 and GPR137B were slightly but statistically significantly changed in MCF-7 cells after exposure to 50 Hz MF (P<0.05), while other analyzed genes exhibited slight up-and down-fluctuations in expressions and no increase or decrease in each gene expression reached statistical significance (P>0.05).
CONCLUSIONThe present study identified three 50 Hz MF responsive genes in MCF-7 cells and the biological consequences of expression changes in these MF responsive genes need to be further investigated.0.4 mT 50 Hz MF exposure for longer duration might induce DNA double-strand breaks in human lens epithelial cells in vitro.
