Bactericidal activity of GLL-37, a novel derivative of the human antimicrobial peptide LL-37.
- Author:
Lin SHI
1
;
Shan LIU
;
Gui-xiang FAN
;
Yu-kang YUAN
;
Long MEI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Bacterial Agents; pharmacology; therapeutic use; Antimicrobial Cationic Peptides; pharmacology; therapeutic use; Blood Bactericidal Activity; drug effects; Cathelicidins; Cell Membrane Permeability; drug effects; Escherichia coli; drug effects; Female; Humans; Membrane Proteins; metabolism; Monocytes; drug effects; Pseudomonas Infections; drug therapy
- From: Journal of Zhejiang University. Medical sciences 2008;37(1):73-77
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop and investigate GLL-37, a substitution analogue of the human antimicrobial peptide LL-37 with anti-enzymatic degradation activity and improved efficacy.
METHODSThe bactericidal activities of LL-37 and newly developed GLL-37 against 6 Gram-negative and -positive bacteria were determined by Broth microdilution assays. The minimum inhibitory concentrations of LL-37 and GLL-37 against E.coli ATCC 25922 in different NaCl concentration medium were also detected. Both peptides were co-incubated with elastase, and then analyzed by PAGE electrophoresis and bactericidal activity determination.
RESULTGLL-37 showed a stronger elastase resistance ability than LL-37, and was significantly more effective than LL-37 under high-salt condition.
CONCLUSIONThe antimicrobial peptide GLL-37 derived form LL-37 has the potential as a new therapeutic agent for bacterial infections.