Alternatively spliced variants of constitutive androstane receptor in liver of mouse.
- Author:
Hong-Ying SUN
1
;
Jing CHEN
;
Kai JIANG
;
Shu-Qing CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Alternative Splicing; Amino Acid Sequence; Animals; DNA, Complementary; genetics; Liver; metabolism; Male; Mice; Molecular Sequence Data; RNA Splice Sites; Receptors, Cytoplasmic and Nuclear; genetics
- From: Journal of Zhejiang University. Medical sciences 2008;37(2):113-117
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the existence of alternatively spliced variants of constitutive androstane receptor (CAR) in liver of mouse.
METHODSThe nucleotide from liver of mouse was purified and the CAR cDNA was amplified by PCR. The fragments of CAR cDNA were cloned to T vector and sequence analysis was performed.
RESULTVarious spliced variants of CAR in liver mouse were confirmed by DNA sequencing.
CONCLUSIONThere are alternatively spliced variants in CAR, which are located in the ligand binding sequence of CAR.
