Inhomogeneous expression of fusion protein HSA/IL1ra in Pichia pastoris.
- Author:
Shou-feng DAI
1
;
Qi SHEN
;
Jing CHEN
;
Shu-qing CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Escherichia coli; genetics; metabolism; Genetic Heterogeneity; Genetic Vectors; genetics; Humans; Interleukin 1 Receptor Antagonist Protein; biosynthesis; genetics; Pichia; genetics; metabolism; Recombinant Fusion Proteins; biosynthesis; genetics; Serum Albumin; biosynthesis; genetics
- From: Journal of Zhejiang University. Medical sciences 2008;37(2):134-138
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the causes and influencing factors of heterogeneity of HSA/IL1ra fusion protein expression in Pichia pastoris.
METHODSThe heterogeneity of HSA/IL1ra fusion protein expressed in Pichia pastoris was studied by removing glycosylation and inhibiting glycosylation, as well as by different ways of fusion, different clones, and different expression host.
RESULTGlycosylation caused expression heterogeneity of fusion protein, but in SMD1168 and some GS115 clones there was no this phenomenon.
CONCLUSIONThe expression heterogeneity of HSA/IL1ra fusion protein in Pichia pastoris is due to the glycosylation, and different ways of fusion, different clones, different expression host also have some impact.
