Luteolin excretion after oral administration of Elsholtzia blanda benth extracts in rats.
- Author:
Jing-yan YING
1
;
Xiang LI
;
Jin-lei WANG
;
Tong-wei YAO
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Animals; Bile; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; metabolism; pharmacokinetics; Feces; chemistry; Lamiaceae; chemistry; Luteolin; isolation & purification; pharmacokinetics; urine; Male; Plant Extracts; administration & dosage; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2008;37(2):139-145
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the excretion of luteolin after oral administration of Elsholtzia blanda benth extracts in rats.
METHODSSamples of urine, feces and bile were collected after oral administration of Elsholtzia blanda benth extracts in rate. After deconjugation with beta-glucuronidase/sulfatase, the levels of luteolin in urine, feces and bile were measured by RP-HPLC.
RESULTThe recovery rate of luteolin was 98.0 %-106.0 % and the extract recoveries were 85.0 %-108.0%. Relative standard deviation (RSD) of intra-and inter-day assay was less than 10.0 %. The total accumulative excretion was 37 % (11 % in urine, 26 % in feces and bile).
CONCLUSIONThe established RP-HPLC method is sensitive, specific, accurate, and is applicable for determination of luteolin in rat urine,feces and bile.
