Expression of telomere binding factor 2 (TRF2) on leukemia cell lines and primary leukemia cells.
- Author:
Xiao-hui CHEN
1
;
Yin TONG
;
Wei-lai XU
;
Jie JIN
;
Wen-bin QIAN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Female; HL-60 Cells; Humans; Jurkat Cells; K562 Cells; Leukemia, Myeloid, Acute; metabolism; Leukemia, T-Cell; metabolism; pathology; Male; Middle Aged; RNA, Messenger; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Telomeric Repeat Binding Protein 2; metabolism; Young Adult
- From: Journal of Zhejiang University. Medical sciences 2008;37(2):170-175
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expression levels of telomere binding factor 2 (TRF2) on leukemia cell lines and primary leukemia cells.
METHODSThe expression of TRF2 mRNA was detected with quantitative real-time RT-PCR in leukemia cell lines and primary leukemia cells. The Western blot analysis was used for the detection of TRF2 protein expression.
RESULTTRF2 was overexpressed in T-cell leukemia cell lines but not in myelogenous leukemia cell lines. Significant higher expression levels of TRF2 were observed in primary leukemia cells from patients with M0 and M1 subtypes of acute myelogenous leukemia (AML) compared with normal control and other subtypes of AML.
CONCLUSIONIncreased TRF2 expression levels are found in T-cell leukemia cell lines and AML patients with poor prognosis, which suggests that TRF2 expression might be related to the prognosis of leukemia.
