Establishment of rabbit model of juvenile nonalcoholic steatohepatitis.
- Author:
Yan-Lan FANG
1
;
Li LIANG
;
Jun-Fen FU
Author Information
- Publication Type:Journal Article
- MeSH: Adiponectin; blood; Animals; Dietary Fats; administration & dosage; Disease Models, Animal; Fatty Liver; blood; metabolism; pathology; Female; Interleukin-10; blood; Male; Rabbits; Random Allocation
- From: Journal of Zhejiang University. Medical sciences 2008;37(3):240-244
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a rabbit model of juvenile nonalcoholic steatohepatitis (NASH) for further study.
METHODSTwenty-eight New Zealand rabbit pups were fed with a high-fat diet (standard diet+10 % lard+2 % cholesterol) for 8 or 12 weeks as the two model groups, and 10 rabbits were fed with standard diet as the controls. Liver tissue samples were collected for Heamatoxylin-Eosin staining and pathological examination.
RESULTTypical histological hepatic lesions of NASH were observed in both model groups. Compared with control group, model groups showed a significant increase in serum ALT, AST, TG, TC levels (P <0.01), and decrease in serum adiponectin, IL-10 levels (P <0.05), meanwhile there was no significant difference between two model groups. TC and the degree of liver fatty infiltration were independent determinants of serum adiponectin level by stepwise multiple regression, beta=-1.33, P=0.006 and beta=-0.97, P=0.038, respectively, R square equal to 0.294.
CONCLUSIONThe juvenile steatohepatitis rabbit model has been established and the level of adiponectin can partly reflect the severity of liver steatosis.