Establishment and characteristics of acute lung injury model induced by cigarette smoke in ICR mice.
- Author:
Qin-chuan WANG
1
;
Yu CHEN
;
Hui-fang TANG
;
Jin-fei TANG
;
Jian-ju LU
;
Ji-qiang CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Acute Lung Injury; chemically induced; pathology; Animals; Bronchoalveolar Lavage Fluid; cytology; Disease Models, Animal; Male; Matrix Metalloproteinase 12; metabolism; Mice; Mice, Inbred ICR; Smoke; adverse effects; Tobacco; adverse effects; Tumor Necrosis Factor-alpha; metabolism
- From: Journal of Zhejiang University. Medical sciences 2008;37(4):328-332
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a mouse model of acute lung injury induced by cigarette smoke (CS) and to investigate inflammatory changes with the model.
METHODSICR mice exposed to CS for 20-min, 3/d. Bronchoalveolar lavage fluid (BALF) and lung tissue were harvested at d 0, d 1, d 3 and d 7 after CS exposure. Neutrophil count in BAFL, TNF-alpha and MMP-12 levels, the activity of MPO in lung tissue were determined.
RESULTNeutrophil count in BALF, MMP-12 and MPO levels in lung tissue were increased after CS exposure in a time-dependent manner with a peak at d3. TNF-alpha level sharply increased at d1, and remained high level until d7.
CONCLUSIONICR mice are tolerant and sensitive to CS exposure, which may be used as an appropriate animal model for acute lung injury induced by cigarette smoke.
