Effect of Cryptoporus polysaccharide on lipopolysaccharide-induced production of monocyte chemoattractant protein-1 in human alveolar epithelial cells.
- Author:
Jianing ZHU
1
;
Hui ZHANG
;
Qiangmin XIE
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Chemokine CCL2; genetics; metabolism; Epithelial Cells; cytology; metabolism; Humans; Lipopolysaccharides; pharmacology; Polyporaceae; chemistry; Polysaccharides; isolation & purification; pharmacology; Pulmonary Alveoli; cytology; RNA, Messenger; metabolism
- From: Journal of Zhejiang University. Medical sciences 2008;37(4):333-339
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of cryptoporus polysaccharide(CP)on lipopolysaccharide(LPS)-induced production of monocyte chemoattractant protein-1(MCP-1)in human lung epithelial A549 cells.
METHODSA549 cells were stimulated with LPS in the presence or absence of CP. The protein concentration and mRNA expression of MCP-1 were determined by enzyme-linked-immunosobent assay(ELISA)and semi-quantitative RT-PCR, respectively.
RESULTThe protein concentration of MCP-1 was significantly increased by LPS 1000 microg/L at 24 h. There were no effects on the growth and viability of A549 cells in the presence of CP 100 microg/L or dexamethasone 1 mumol/L. However, CP 100 microg/L or dexamethasone 1 micromol/L significantly inhibited the protein concentration and mRNA expression of MCP-1 induced by LPS.
CONCLUSIONCP can regulate MCP-1 production, which may be associated with its effects on lung inflammation.
