Neuron regeneration in aged rats after intracerebral hemorrhage.
- Author:
Shu-xu YANG
1
;
Yi-rong WANG
;
Cong QIAN
;
Chao HE
Author Information
- Publication Type:Journal Article
- MeSH: Age Factors; Animals; Cerebral Hemorrhage; physiopathology; Male; Nerve Regeneration; physiology; Neuronal Plasticity; physiology; Neurons; physiology; Rats; Rats, Sprague-Dawley
- From: Journal of Zhejiang University. Medical sciences 2008;37(4):386-392
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate neuron regeneration of aged rats after intracerebral hemorrhage(ICH).
METHODSICH model was induced by intracerebral infusion of 100microL autologous blood in adult and aged (3 and 18 m of age, respectively) male Sprague-Dawley (SD) rats. Doublecortin(DCX), polysialylated neural cell adhesion protein(PSA-NCAM) and Bromodeoxyuridine (Brdu) in brain tissue were detected by Western blot analysis and immunohistochemical examination.
RESULTIn aged rats, DCX levels in the ipsilateral basal ganglia and subventricular zone started to increase at 7d after ICH, peaked at 14 d [(670+/-145)% of controls, P<0.01]. Most DCX positive cells in ipsilateral striatum were BrdU positive. Though DCX content in ipsilateral basal ganglia [(2560+/-758) pixels]of ICH aged rats was much higher than that of aged rat controls [(450+/-97)pixels, P<0.01], it was still lower than that in adult rats.
CONCLUSIONThe results demonstrate that neuron regeneration occurs in subventricular zone in the aged rats following intracerebral hemorrhage(ICH).
