Analysis of SLC25A13 gene mutations in five infants with neonatal intrahepatic cholestasis caused by citrin deficiency.

VernacularTitle:五例新生儿肝内胆汁淤积症患儿SLC25A13基因的突变分析
Author: Junjie XU ¹ ; Min GAO ; Yuqiang LYU ; Yunping TANG ; Xuxia WEI ; Lu YANG ; Kaihui ZHANG ; Yi LIU ; Zhongtao GAI
Author Information

1. Department of Gastroenterology, Jinan Institute of Pediatric Research, Qilu Children's Hospital of Shandong University, Jinan, Shandong 250022, China. Email: gaizhongtao@sina.com.
Publication Type:Journal Article
From: Chinese Journal of Medical Genetics 2018;35(1):34-38
CountryChina
Language:Chinese
Abstract: OBJECTIVE To identify potential mutations in five infants with neonatal intrahepatic cholestasis caused by citrin deficiency (NICCD). METHODS The SLC25A13 gene was analyzed by next-generation sequencing. Suspected mutations were confirmed by PCR and Sanger sequencing in the probands and their parents. Impact of novel mutations was predicted with PolyPhen-2 software. RESULTS All neonates have harbored mutations of the SLC25A13 gene. Eight mutations were discovered, which included two novel mutations (c.1357A>G and c.1663dup23). All parents were found to be carriers of the mutations. CONCLUSION Mutations of the SLC25A13 gene probably underlie the NICCD among the five patients, among which 851del4 and 1638-1660dup were the most common ones. This has enriched the spectrum of SLC25A13 mutation in association with NICCD.