Paeonol attenuates oxygen-glucose deprivation injury and inhibits NMDA receptor activation of cultured rat hippocampal neurons.
- Author:
Ning-ning SONG
1
;
Ji-biao WU
;
Xin-bing WEI
;
Hua-shi GUAN
;
Xiu-mei ZHANG
Author Information
1. Qilu Hospital, Shandong University, Jinan 250012, China.
- Publication Type:Journal Article
- MeSH:
Acetophenones;
isolation & purification;
pharmacology;
Animals;
Cell Hypoxia;
Cell Survival;
drug effects;
Cells, Cultured;
Dizocilpine Maleate;
pharmacology;
Glucose;
deficiency;
Glutamic Acid;
metabolism;
Hippocampus;
cytology;
Neurons;
cytology;
Neuroprotective Agents;
isolation & purification;
pharmacology;
Paeonia;
chemistry;
Plants, Medicinal;
chemistry;
Protein Binding;
RNA, Messenger;
metabolism;
Random Allocation;
Rats;
Rats, Wistar;
Receptors, N-Methyl-D-Aspartate;
genetics;
metabolism
- From:
Acta Pharmaceutica Sinica
2009;44(11):1228-1232
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of this study is to determine if paeonol can protect hippocampal neurons against injury due to oxygen-glucose deprivation (OGD) injury. The rat neurons were cultured in an OGD environment and the model of OGD injury was established. Paeonol and MK-801, a positive control drug, were added before deprivation. Neuron viability was measured by the reduction of MTT; glutamate was analyzed by amino acid analyzer; binding activity of NMDA receptor was evaluated by liquid scintillation counting and the expression of NMDA receptor NR1 subunit mRNA was semiquantitatively determined by RT-PCR. Compared with OGD injury group, paeonol treatment obviously increased cell survival rate and reduced the binding activity of NMDA receptors and the release of glutamate; and down-regulating the expression of NR1 subunit. These results suggest that paeonol may exhibit its protective effect against OGD injury by the action on NMDA receptor of rats.
