Preparation of brain targeted immunoliposomes.
- Author:
Hao ZHAO
1
;
Ren-zhi WANG
;
Fei WANG
;
Yan-hui ZHANG
;
Xing-wei CHEN
;
Xin-ru LI
;
Yan LIU
;
Gui-lin LI
;
Jun-ji WEI
;
Ming FENG
;
Yan-guo KONG
;
Shi-fang LI
Author Information
1. Department of Neurosurgery, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies, Monoclonal;
administration & dosage;
pharmacokinetics;
Blood-Brain Barrier;
Brain;
blood supply;
immunology;
metabolism;
Drug Delivery Systems;
methods;
Genetic Vectors;
Lac Operon;
genetics;
Liposomes;
administration & dosage;
immunology;
pharmacokinetics;
Male;
Particle Size;
Plasmids;
Polyethylene Glycols;
administration & dosage;
pharmacokinetics;
Rats;
Receptors, Transferrin;
immunology;
Tissue Distribution;
beta-Galactosidase;
genetics;
metabolism
- From:
Acta Pharmaceutica Sinica
2009;44(11):1285-1290
- CountryChina
- Language:Chinese
-
Abstract:
To prepare a kind of effective non-viral transduction vector, which can deliver exogenous gene into the brain, this vector can be injected through vein system and has the ability to penetrate blood brain barrier. Several groups of materials proportion, type of oil phase, water-oil ratio, phosphatides-cholesterol ratio, temperature of steaming, ultrasonic temperature and time were compared for optimization. Well-constructed immunoliposomes encapsuling LacZ gene were infused into rats through tail vein. 48 h after injection, expression product beta-galactosidase of LacZ gene was detected by histochemistry staining to convince the validity of immunoliposomes as non-viral vectors. The best proportion of synthesis immunoliposomes is as following: phosphatides-cholesterol ratio is 1:1, lipids/drug is 100:1, the type of oil phrase is dichloromethane, oil-water ratio is 4:1, temperature of steaming is 30 degrees C, ultrasonic temperature and time is 10 degrees C and 5 min. At last, 10% trehalose was added as a stabilizer. The entrapment rate is 87.24% and antibody coupling rate is 69%. When immunoliposomes were infused into rats, the expression of LacZ gene could be observed in the brain and periphery organs. Through the best proportion of materials, gene delivering immunoliposomes had been synthesized successfully. This non-viral vector can deliver exogenous gene penetrating blood brain barrier and express in the brain, and will be well-used in the field of gene therapy of cerebral diseases.
