8-hydroxy-dihydroberberine ameliorated insulin resistance induced by high FFA and high glucose in 3T3-L1 adipocytes.
- Author:
Li-jun XU
1
;
Fu-er LU
;
Ping YI
;
Zeng-si WANG
;
Shi-chao WEI
;
Guang CHEN
;
Hui DONG
;
Xin ZOU
Author Information
1. Institute of Integrated Traditional Chinese and Western Medicine, Huazhong University of Science and Technology, Wuhan 430030, China.
- Publication Type:Journal Article
- MeSH:
3T3-L1 Cells;
Adipocytes;
cytology;
drug effects;
metabolism;
Animals;
Berberine;
analogs & derivatives;
chemical synthesis;
chemistry;
pharmacology;
Cell Differentiation;
drug effects;
Glucose;
metabolism;
pharmacology;
Hypoglycemic Agents;
chemical synthesis;
chemistry;
pharmacology;
Insulin;
pharmacology;
Insulin Resistance;
Mice;
Molecular Structure;
Palmitic Acid;
pharmacology
- From:
Acta Pharmaceutica Sinica
2009;44(11):1304-1308
- CountryChina
- Language:Chinese
-
Abstract:
The purpose of the study is to investigate the effect of 8-hydroxy-dihydroberberine on insulin resistance induced by high free fatty acid (FFA) and high glucose in 3T3-L1 adipocytes and its possible molecular mechanism. Palmic acid or glucose in combination with insulin was used to induce insulin resistance in 3T3-L1 adipocytes. 8-Hydroxy-dihydroberberine and berberine were added to the cultured medium separately, which were considered as treated group and positive control group. The rate of glucose uptake was determined by 2-deoxy-[3H]-D-glucose method. The amount of glucose consumption in the medium was measured by glucose oxidase method. Cell growth and proliferation of 3T3-L1 adipocytes were detected with Cell Counting Kit-8 (CCK-8) assay. After incubated with palmic acid for 24 hours or glucose with insulin for 18 hours, the rate of glucose transport in 3T3-L1 adipocytes was inhibited by 67% and 58%, respectively. The amount of glucose consumption in 3T3-L1 adipose cells was decreased by 41% after cells were incubated with palmic acid for 24 h. However, the above changes were reversed by pretreatment with 8-hydroxy-dihydroberberine for 24 and 48 h. Significant difference existed between groups. Insulin resistance in 3T3-L1 adipocytes, which is induced by high FFA and high glucose, could be ameliorated by 8-hydroxy-dihydroberberine.
