Anti-tumor activity of Erythrina variegata L. extract and its mechanism of action.
- Author:
Hong ZHANG
1
;
Jun-Feng XIANG
;
Li TAN
;
Wei DAI
;
Ge BAI
;
Yan LIU
;
Xue-Li CAO
;
Ya-Lin TANG
Author Information
1. State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antineoplastic Agents, Phytogenic;
isolation & purification;
pharmacology;
Carcinoma, Lewis Lung;
pathology;
Cell Line, Tumor;
Cell Proliferation;
drug effects;
Drugs, Chinese Herbal;
isolation & purification;
pharmacology;
Erythrina;
chemistry;
G-Quadruplexes;
drug effects;
Humans;
Male;
Mice;
Mice, Inbred C57BL;
Plants, Medicinal;
chemistry;
Tumor Burden;
drug effects
- From:
Acta Pharmaceutica Sinica
2009;44(12):1359-1363
- CountryChina
- Language:Chinese
-
Abstract:
The anti-tumor activities and mechanism of Erythrina variegata L. extract were investigated. Firstly, the MTT method was used to evaluate the inhibitory activity of the Erythrina variegata L. extract on proliferation of cancer cell lines. Moreover, in order to determine its anti-tumor effect in vivo, the Lewis lung cancer mice model was established. By comparing the relative tumor proliferation rates, growth curves, inhibition rates of different groups, the anti-tumor effect was evaluated. Furthermore, the anti-tumor mechanism of Erythrina variegata L. extract was studied by using G-quadruplex stability experiment. In the in vitro anti-liver cancer experiment, the Erythrina variegata L. extract has shown obvious anti-tumor effect on various tumor cells. And in the in vivo experiment, it exhibited significant anti-tumor effect. Besides, from the result of G-quadruplex stability experiment, we can see that the quadruplex structure show increasing T(m) values with increasing amounts of Erythrina variegata L. extract.
