Quantification of pentoxyverine citrate in human plasma by LC-ESI/MS method and its application.
- Author:
Ke-Hua YU
1
;
Jin-Hua WEN
;
Fu-Gen NIE
;
Yu-Qing XIONG
Author Information
1. Department of Experimental Teaching, College of Medicine, Nanchang University, Nanchang 330006, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, Liquid;
methods;
Cyclopentanes;
blood;
pharmacokinetics;
Humans;
Male;
Sensitivity and Specificity;
Spectrometry, Mass, Electrospray Ionization;
methods;
Therapeutic Equivalency
- From:
Acta Pharmaceutica Sinica
2009;44(12):1402-1405
- CountryChina
- Language:Chinese
-
Abstract:
A rapid and sensitive liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI/ MS) method for quantification of pentoxyverine citrate in human plasma has been developed and applied for the bioequivalence and pharmacokinetics study. After extracted from plasma samples with ethyl acetate, analysis was performed in selected ion monitoring (SIM) mode with a positive electrospray ionization (ESI) interface with a mobile phase consisted of methanol and water (0.4% glacial acetic acid and 4 mmol x L(-1) ammonium acetate, 43 : 57, v/v). The linear concentration range of the calibration curves was 1.0-160.0 ng x mL(-1) for pentoxyverine citrate, inter- and intra-precision (RSD) was less than 12.5%, accuracy (RE) was in +/- 13.5% and absolute recovery was more than 80%. The method was proved simple, rapid, sensitive, specific and suitable for pharmacokinetic and bioequivalence study of Yufenweilin capsule containing pentoxyverine citrate.
