Effect of curcumin on JAK-STAT signaling pathway in hepatoma cell lines.
- Author:
Wei-Zhang WANG
1
;
Bi-Yu ZHANG
;
Jian YUAN
;
Jian-Wen MAO
;
Wen-Jie MEI
Author Information
1. Guangdong Pharmaceutical University, Guangzhou 510006, China. wwzss@163.com
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents, Phytogenic;
isolation & purification;
pharmacology;
Apoptosis;
Carcinoma, Hepatocellular;
metabolism;
pathology;
Cell Line, Tumor;
Cell Proliferation;
Curcuma;
chemistry;
Curcumin;
isolation & purification;
pharmacology;
Humans;
Janus Kinases;
metabolism;
Liver Neoplasms;
metabolism;
pathology;
Phosphorylation;
Plants, Medicinal;
chemistry;
STAT1 Transcription Factor;
metabolism;
Signal Transduction;
p38 Mitogen-Activated Protein Kinases;
metabolism
- From:
Acta Pharmaceutica Sinica
2009;44(12):1434-1439
- CountryChina
- Language:Chinese
-
Abstract:
The effect of curcumin on JAK-STAT signaling pathway was investigated in hepatoma cell lines Huh7 and Hep3B. Curcumin inhibited cell proliferation and induced apoptosis of both cell lines, but Huh7 cells were more sensitive to curcumin than Hep3B cells. Curcumin (50 micromol x L(-1)) significantly increased phosphorylations of p38 (T180/Y182) and STAT-1 (S727) in Huh7 and Hep3B cells, and caused relocalization of phosphorylated-STAT-1 (Y701) from cytoplasm to nucleus in Hep3B cells. In addition, curcumin (25 and 50 micromol x L(-1)) dramatically suppressed the phosphorylation level of STAT-1 (Y701) and resulted in a significant reduction of nuclear phosphorylated-STAT-1 (Y701) in Huh7 cells.
