Effects of hyperin on the cccDNA of duck hepatitis B virus and its immunological regulation.
- Author:
Miao GENG
1
;
Jian-Hua WANG
;
Hong-Yan CHEN
;
Xin-Bo YANG
;
Zheng-Ming HUANG
Author Information
1. Institute of Geriatrics, Chinese PLA General Hospital, Beijing 100853, China. gengmiao_2000@yahoo.com
- Publication Type:Journal Article
- MeSH:
Animals;
Antiviral Agents;
pharmacology;
DNA, Circular;
metabolism;
DNA, Viral;
metabolism;
Hepadnaviridae Infections;
virology;
Hepatitis B Virus, Duck;
genetics;
Hepatitis, Viral, Animal;
virology;
Interferon-gamma;
secretion;
Interleukin-12;
secretion;
Liver;
virology;
Lymphocytes;
secretion;
Mice;
Quercetin;
analogs & derivatives;
pharmacology;
Spleen;
pathology;
virology
- From:
Acta Pharmaceutica Sinica
2009;44(12):1440-1444
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to investigate the effect of hyperin on the cccDNA of duck hepatitis B virus and its immunological regulation. Duck hepatitis B virus (DHBV) infection model and normal mouse spleen lymphocyte were used to evaluate the anti-HBV and immunoregulation effects. The DHBV-DNA of serum was detected at different time points by using serum DOT-BLOT hybridization. Polymerase chain reaction (PCR) was used for the determination of nuclear covalent closed circular DNA (cccDNA). Cytokine secretion was determined by ELISA method. DHBV-DNA were inhibited by hyperin (25 or 50 mg x kg(-1)), while cccDNA of liver could be eliminated efficiently by hyperin (25 or 50 mg x kg(-1), P < 0.05, P < 0.01). The T helper 1 effector cytokine was markedly enhanced by hyperin (25 or 50 microg x mL(-1), P < 0.01). In conclusion, hyperin has anti-HBV activity via multiple targets and pathways, and cccDNA may be one of the important targets.
