Advances in the study of affinity selection-ultrafiltration/HPLC-MS.
- Author:
Hong-Yan ZHAO
1
;
Yong-Zhong ZHANG
;
Chun-Ling XIAO
Author Information
1. Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
methods;
Combinatorial Chemistry Techniques;
Drug Evaluation, Preclinical;
methods;
Humans;
Ligands;
Mass Spectrometry;
methods;
Pharmaceutical Preparations;
metabolism;
Protein Binding;
Small Molecule Libraries;
Ultrafiltration;
methods
- From:
Acta Pharmaceutica Sinica
2009;44(10):1084-1088
- CountryChina
- Language:Chinese
-
Abstract:
Affinity selection-ultrafiltration/HPLC-MS is the combination of the ultrafiltration and HPLC-MS, mainly used for screening small active molecular substances from combinatorial libraries and natural product extracts, which can bind to solution-phase targets. Besides, it can be used in metabolic screening and characterization of ligand-receptor binding. It is a complement to the traditional methods of screening and identifying drugs. This review describes its principle and application in drug study.