Effect of p21-targeted shRNA on curcumin-induced apoptosis of human hepatoma Huh7 cells.
- Author:
Wei-Zhang WANG
1
;
Bi-Yu ZHANG
;
Wen-Jie MEI
;
Jian-Wen MAO
;
Ming LI
Author Information
1. Guangdong Pharmaceutical University, Guangzhou 510006, China. wwzss@163.com
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Apoptosis;
drug effects;
Carcinoma, Hepatocellular;
metabolism;
pathology;
Cell Line, Tumor;
Curcumin;
pharmacology;
Cyclin-Dependent Kinase Inhibitor p21;
genetics;
metabolism;
Gene Expression Regulation, Neoplastic;
Humans;
Liver Neoplasms;
metabolism;
pathology;
Plasmids;
RNA, Messenger;
metabolism;
RNA, Small Interfering;
genetics;
Transfection;
Up-Regulation
- From:
Acta Pharmaceutica Sinica
2009;44(10):1102-1106
- CountryChina
- Language:Chinese
-
Abstract:
In the present study, shRNA plasmid of pSi-p21 targeting p21 mRNA was constructed and the effect of p21 shRNA on curcumin-induced apoptosis of human hepatoma Huh7 cells was investigated. The effect of curcumin on the expression of p21 mRNA and protein and the silence efficiency of pSi-p21 were detected with RT-PCR and Western blotting. The effect of pSi-p21 on curcumin-induced apoptosis of Huh7 cells was evaluated with DAPI staining. The results showed that curcumin significantly upregulated p21 mRNA and protein expression, which was knocked down by pSi-p21 of Huh7 cells. DAPI staining results showed that pSi-p21 significantly decreased curcumin-induced apoptosis of Huh7 cells. The data suggested that curcumin induced apoptosis of Huh7 cells via upregulation of p21 expression.
