Determination of colistin based on biothermal activity detection method.
- Author:
Yun LUO
1
;
Dan YAN
;
Yong-Shen REN
;
Shao-Feng ZHANG
;
Xue FENG
;
Han-Bing LI
;
Hui-Ying TANG
;
Xiao-He XIAO
Author Information
1. China Military Institute of Chinese Materia Medica, 302 Military Hospital of China, Beijing 100039, China.
- Publication Type:Journal Article
- MeSH:
Anti-Bacterial Agents;
administration & dosage;
pharmacology;
Calorimetry;
methods;
Colistin;
administration & dosage;
pharmacology;
Dose-Response Relationship, Drug;
Escherichia coli;
drug effects;
growth & development;
Microbial Sensitivity Tests;
methods;
Thermodynamics
- From:
Acta Pharmaceutica Sinica
2009;44(10):1136-1139
- CountryChina
- Language:Chinese
-
Abstract:
A biothermal activity detection method has been established to determine the potency of colistin. The biothermal activity fingerprints of E. coli with colistin were determined. There was a good linear relationship (r = 0.993) between logarithm concentration of colistin (lgC) and lag rate of growing time (Deltat%) when the concentrations of colistin ranged from 17.0 to 41.6 u x mL(-1). The average recovery rate was 100.3% (n = 9). Using this method, there was no significant difference between results of colistin potency measurement and those using cup-plate method (P > 0.05). As a result, biothermal activity detection method is sensitive, accurate, rapid, convenient and feasible to determine the potency of colistin. This method can also be applied in real time and online to monitor the process of bacterial growth and could be complementary to the cup-plate method.
