Determination of icaritin in rat plasma by HPLC-MS/MS.
- Author:
Hai-Pei LIU
1
;
Fan-Hua MENG
;
Ji-Fen GUO
;
Duan-Yun SI
;
Xiao-Wei ZHU
;
Yi-Min ZHAO
Author Information
1. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Administration, Oral;
Animals;
Chromatography, High Pressure Liquid;
methods;
Epimedium;
chemistry;
Female;
Flavonoids;
administration & dosage;
blood;
isolation & purification;
pharmacokinetics;
Male;
Plants, Medicinal;
chemistry;
Rats;
Rats, Wistar;
Reproducibility of Results;
Sensitivity and Specificity;
Spectrometry, Mass, Electrospray Ionization;
methods;
Tandem Mass Spectrometry;
methods
- From:
Acta Pharmaceutica Sinica
2009;44(10):1140-1144
- CountryChina
- Language:Chinese
-
Abstract:
The paper is to report the development of a high-performance liquid chromatographic/tandem mass spectrometry (HPLC-MS/MS) method for the determination of icaritin (ICT) in rat plasma. After precipitated with acetonitrile from the plasma, ICT was isolated chromatographically on a Dikma C18 column. The mobile phase consisted of acetonitrile-water-acetic acid (72 : 28 : 1.5, v/v/v). Electrospray ionization (ESI) source was applied and operated in the positive ion mode. Multiple reaction monitoring (MRM) mode with the transitions of m/z 387 --> m/z 313 and m/z 331 --> m/z 315 were used to quantify ICT and the internal standard, respectively. The linear calibration curve was obtained in the concentration range of 2.5-1,000 ng x mL(-1). The lower limit of quantification was 2.5 ng x mL(-1). The inter- and intra-day precision (RSD) were less than 9.63%, and the accuracy (relative error) was within +/-7.42%. The method was proved to be suitable for the pharmacokinetics of ICT, which offers advantages of high sensitivity and selectivity.
