Effects of extracts of cheezheng pain relieving plaster on nitric oxide and iNOS expression in macrophages induced by lipopolysaccharides.
- Author:
Yang LIU
1
;
Shan-Ying PENG
;
Lin WANG
;
Wen-Jie WANG
Author Information
1. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Drugs, Chinese Herbal;
pharmacology;
Lipopolysaccharides;
adverse effects;
Macrophages;
drug effects;
metabolism;
Male;
Mice;
Mice, Inbred C57BL;
Nitric Oxide;
metabolism;
Nitric Oxide Synthase Type II;
metabolism;
RNA, Messenger;
genetics;
Transcription Factor RelA;
metabolism
- From:
Acta Pharmaceutica Sinica
2009;44(8):863-867
- CountryChina
- Language:Chinese
-
Abstract:
This study is to explore the effects of extracts of Cheezheng pain relieving plaster (ECPRP) on nitric oxide (NO) production and expression of inducible nitric oxide synthase (iNOS) in macrophages induced by LPS and the mechanism involved. Nitric oxide level was measured with Griess reagent assay. Inducible nitric oxide synthase (iNOS) protein and NF-kappaBp65 fragment were detected with Western blotting. ECPRP (62.5 and 125 mgL(-1)) significantly inhibited the increase of nitric oxide level. Furthermore, ECPRP (62.5 and 125 mg x L(-1)) notably reduced the expression of iNOS mRNA and protein. ECPRP (62.5 and 125 mg x L(-1)) elevated the content of I-kappaB protein in cytoplasm, while decreased the content of NF-kappaBp65 protein in nucleus. These results suggest that ECPRP reduce nitric oxide level via down-regulation of NF-kappaB-iNOS-nitric oxide pathway, resulting in prevention of inflammation.
