Analysis of the relationship between Glut-4 gene polymorphism and blood-fat traits in induced hyperglycemia mice.
- Author:
Qin CHEN
1
;
Wen-Jun WANG
;
Xin-Chen SHANG-GUAN
;
Ming-Sheng XU
Author Information
1. College of Food Science and Engineering, Jiangxi Agricultural University, Nanchang 330045, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Glucose Transporter Type 4;
genetics;
Hyperglycemia;
blood;
genetics;
Lipids;
blood;
Mice;
Mice, Obese;
genetics;
Polymorphism, Single Nucleotide
- From:
Acta Pharmaceutica Sinica
2009;44(8):931-934
- CountryChina
- Language:Chinese
-
Abstract:
This study is to investigate the effect of diet and gene on blood-fat trait of the individual mouse. One hundred and twenty mice were fed with high fat emulsion for 4 weeks. Then the genotypes of Glut-4 were analyzed by PCR-SSCP to investigate the effect on blood-fat traits and some organ performance of mice. After fed with high fat emulsion for 4 weeks, 98 mice suffered from hyperlipidemia, while 22 mice did not. And a T/A mutation was found in 188 targeted fragments we amplified at 8,521 site, and association analysis showed that the mice carried BB genotype had higher TC, TG and LW/BW level than those of the mice with AA genotype (P < 0.05). Different Glut-4 genotypes show different sensitivities to high fat emulsion on mice.
