Effect of guilu erxianjiao in suppressing splenic T-lymphocyte apoptosis in mice undergoing chemotherpy.
- Author:
Sheng-You LIN
1
;
Min-He SHEN
;
Jian CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; drug effects; Caspase 3; genetics; metabolism; Cell Proliferation; drug effects; Drugs, Chinese Herbal; administration & dosage; Humans; Male; Mice; Neoplasms; drug therapy; genetics; metabolism; physiopathology; Proto-Oncogene Proteins c-bcl-2; genetics; metabolism; Random Allocation; Spleen; cytology; drug effects; metabolism; T-Lymphocytes; cytology; drug effects; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2008;28(4):339-342
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Guilu Erxianjiao (GLEXJ) in suppressing chemotherapy induced cell apoptosis in mice and to discuss its possible acting mechanism.
METHODSSeventy-two tumor-bearing Kunming mice were randomly divided into 6 groups, Group A, treated with normal saline; Group B treated with cyclophosphamide (CTX), Group C treated with Chinese herbal medicine, and Group D-F, the three combined treated group received CTX plus high, moderate and low dose GLEXJ. All mice were sacrificed after 9-day medication, their splenic tissues were taken for determining T-lymphocyte apoptosis with flow cytometer, the expression of bcl-2 mRNA and Caspase-3mRNA detected by RT-PCR.
RESULTSAs compared with Group A, FITC+/PI- cell proportion of spleen T-lymphocyte cells and Caspase-3mRNA expression were higher, while bcl-2 mRNA expression was lower in Group B; as compared with Group B, the former two indexes were lower and the latter was higher in the three combined treated groups (all P <0.05).
CONCLUSIONGLEXJ could efficiently suppress the splenic T-lymphocyte apoptosis in tumor bearing mice undergoing chemotherapy, one of its mechanisms may be through up-regulating of bcl-2 mRNA expression and down-regulating of Caspase-3mRNA expression.
