Study of modified Biejiajian pill on pathological immune hepatic fibrosis model induced by albumin in rats.
- Author:
Hai-Yun SUN
1
;
Ying REN
;
Jun-Xiang YIN
Author Information
- Publication Type:Journal Article
- MeSH: Albumins; pharmacology; Animals; Collagen; metabolism; Disease Models, Animal; Dose-Response Relationship, Drug; Drugs, Chinese Herbal; pharmacology; Humans; Liver; drug effects; metabolism; pathology; Liver Cirrhosis; chemically induced; immunology; metabolism; pathology; Membrane Glycoproteins; metabolism; Microfilament Proteins; metabolism; Rats; Rats, Wistar; Spleen; drug effects; immunology
- From: Chinese Journal of Integrated Traditional and Western Medicine 2008;28(6):526-528
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo quantitatively analyze the effect of modified Biejiajian Pill (BJJP) on the pathological change and degree of albumin induced immune hepatic fibrosis in rats.
METHODSRats were immunized by multiple subcutaneous injections of human serum albumin (8 g/L) , and were medicated in groups respectively after antibody producing, BJJP high-dose (13 g/kg) group, medium-dose (6.5 g/kg) group, low-dose (3.25 g/kg) group, the model group, colchicines (1.0 mg/kg) group, and Ganpikang (22.23 mg/kg) group. Then, caudal vein injection of albumin was given 40 min after medication to induce liver fibrosis. Animals were sacrificed finally to observe the pathological change, and the distribution and content of collagen and plastin were determined quantitatively with HE and Masson stain.
RESULTSBJJP high-, medium-, and low-dose groups could obviously improve the pathological change of the hepatic fibrosis rats (decreasing rate of the total score was 62.50%, 40.75%, and 8.33%, respectively), and the content of collagen reduced markedly (P<0.05, P<0.01).
CONCLUSIONBJJP can effectively prevent and reduce the pathological change of albumin induced immune hepatic fibrosis in rats.
