Effect of ethyl acetate extract of sappan wood on expression of myocardial GrB mRNA in rat model of allogeneic ectopic cardiac transplantation.
- Author:
Jia-Xin ZHENG
1
;
Ya-Bin ZHOU
;
Ying-Zhe LIU
Author Information
- Publication Type:Journal Article
- MeSH: Acetates; chemistry; Animals; Caesalpinia; chemistry; Gene Expression Regulation, Enzymologic; drug effects; Graft Rejection; drug therapy; Granzymes; genetics; Heart; drug effects; Heart Transplantation; immunology; Male; Models, Animal; Myocardium; metabolism; pathology; Plant Extracts; pharmacology; therapeutic use; RNA, Messenger; genetics; metabolism; Rats; Transplantation, Homologous
- From: Chinese Journal of Integrated Traditional and Western Medicine 2008;28(6):537-540
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the action mechanism of the immune active components of sappon wood (SWE) for antagonizing reject reaction by observing the influence of its ethyl acetate extract on mRNA expression of myocardial GrB in rat model of allogenic ectopic cardiac transplantation.
METHODSAnimal model of abdominal cardiac ectopic transplantation was established taking Wistar rat as the donor and SD rat as the receptor. The successfully modeled rats were randomly divided into the model group, the SWE group and the CsA group. GrB mRNA expression was detected by RT-PCR method and myocardial pathomorphologic picture was observed in routine.
RESULTSThe pathologic changes in the SWE group (23 scores) and the CsA group (14 scores) were milder than in the model group (31 scores), the former two could markedly alleviate the myocardial pathologic injury (P<0.05, P<0.01). The GrB mRNA expression in the model group was 1.3000 +/- 0.1207, the SWE group 0. 7070 +/- 0.1215, and the CsA group 0.6700 +/- 0.0997, respectively; compared with the model group, the latter two could obviously down-regulate the expression of GrB mRNA (P<0.01) and no significant difference was found between the latter two groups (P>0.05).
CONCLUSIONSWE could alleviate the pathologic change, down-regulate the mRNA expression of myocardial GrB in allogenic ectopic transplanted myocardium of rats, it is possibly one of the factors for its antagonizing effect against reject reaction.
