Study on the expansion of megakaryocyte progenitors in vitro from cord blood.
- Author:
Guo-Hua CHEN
1
;
Jian-Pei FANG
;
Hong-Gui XU
;
Si-Xi LIU
;
Shao-Liang HUANG
Author Information
1. Department of Pediatrics, Huizhou Central Hospital, Huizhou, China.
- Publication Type:Journal Article
- MeSH:
Antigens, CD34;
analysis;
Blood Platelets;
cytology;
immunology;
Cell Differentiation;
drug effects;
Cell Proliferation;
drug effects;
Cells, Cultured;
Fetal Blood;
cytology;
immunology;
Humans;
Interleukin-3;
pharmacology;
Interleukin-6;
pharmacology;
Megakaryocytes;
cytology;
immunology;
Membrane Proteins;
pharmacology;
Platelet Membrane Glycoprotein IIb;
analysis;
Stem Cells;
cytology;
immunology;
Thrombopoietin;
pharmacology
- From:
Journal of Experimental Hematology
2005;13(4):660-663
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the effect of various cytokines on megakeryocytes expansion in vitro from human cord blood CD34(+) cells in order to establish an optimal culture system for MK expansion. Mononuclear cells were obtained by Ficoll-Hapaque density gradient separation. CD34(+) cells were positively isolated using a CD34 progenitor cell isolation kit. CD34(+) cells were placed into 24 well plates at a concentration of 2 x 10(4) per well. Each well contained 1 ml of IMDM with the present of effective MK cells growth cytokines. Clonogenic potentials of MK progenitor were assayed using a methylcellulose cultures system. The results suggested that four cytokines (IL-3 + IL-6 + TPO + FLT3L) culture system could effectively induce and expand cord blood CD41(+) MK cells. The number of CD41(+) cells expanded 154.67 +/- 32.21-fold on day 7, and 193.23 +/- 25.24-fold on day 14. In conclusion, established expansion system in vitro for MK cells provides experimental foundation for recovery of platelets after cord blood transplantation.
