Gene expression profile in K562 cells treated by interferon alpha.
- Author:
Bin WU
1
;
Shu-Yun ZHOU
;
Xiao-Li LIU
Author Information
1. Department of Hematology, Nan Fang Hospital, The First Military Medical University, Guangzhou, China.
- Publication Type:Journal Article
- MeSH:
Gene Expression Profiling;
Gene Expression Regulation, Neoplastic;
drug effects;
Humans;
Interferon-alpha;
pharmacology;
Janus Kinase 1;
genetics;
K562 Cells;
Oligonucleotide Array Sequence Analysis;
STAT1 Transcription Factor;
genetics;
STAT2 Transcription Factor;
genetics
- From:
Journal of Experimental Hematology
2005;13(5):746-750
- CountryChina
- Language:Chinese
-
Abstract:
To study the gene expression profile in K562 cells treated by IFN-alpha, so as to provide some information about the potential mechanism of IFN-alpha curing CML, the changes of gene expression were examined with the DNA array in K562 cells before and after treatment with IFN-alpha. The results showed that no gene expression difference more than 2.5 times in K562 cells was found on the first day after treatment with IFN-alpha (200 U/ml), then the genes significant expression difference increased step by step, and reached the peak on the forth day. In all examined genes, 97 genes significant expression difference were detected, 86.60% (84/97) gene of interest out of those gene were up-regulated, 13.40% (13/97) were down-regulated. In these 97 genes with significant expression difference, cell regulator protein genes accounted to 23.71% (23/97), surface receptor genes 14.43% (14/97), oncogenes and tumor suppressors 11.34% (11/97), extracellular communication proteins 9.28% (9/97), cell adhesion molecular genes 8.25% (8/97) and the other genes accounted to 32.99% (32/97). JAK1 was up-regulated to 3.78 times, JAK2 to 15.43, STAT1 and STAT2 were up-regulated to 11.98 and 8.11 times respectively, and these genes are components of JAK-STAT pathway. The number of different genes began to decrease on the fifth day. There were still 9 genes that had expression difference more than 3 times on the twenty-first day. It is concluded that when concentration of IFN-alpha was 200 U/ml, the forth day should be considered as the best time to examine change of gene expression in K562 cells treated by IFN-alpha. IFN-alpha realizes its biological functions through the JAK-STAT pathways and it may be one of the mechanisms for curing CML with IFN-alpha.
