Conventional cytogenetics and fluorescence in situ hybridization as methods for detecting MLL gene rearrangements in leukemia.
- Author:
Xu-Ping LIU
1
;
Cheng-Wen LI
;
Shuang QIN
;
Yun DAI
;
Ji-Gang XIAO
;
Qi HUANG
;
Fang-Yun XU
;
Jin-Ying GONG
;
Shi-He LIU
Author Information
1. Institute of Hematology, Detection Center of Hematological Hospital, China Union Medical University, Chinese Academy of Medical Sciences, Tianjin 300020 China. ZIZHAO@UNIONHOPE.COM
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Child;
Child, Preschool;
Chromosome Aberrations;
Chromosome Banding;
Chromosomes, Human, Pair 11;
genetics;
Female;
Gene Rearrangement;
Histone-Lysine N-Methyltransferase;
Humans;
In Situ Hybridization, Fluorescence;
methods;
Infant;
Karyotyping;
Leukemia;
genetics;
Male;
Middle Aged;
Myeloid-Lymphoid Leukemia Protein;
genetics
- From:
Journal of Experimental Hematology
2005;13(5):798-803
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to compare the values of conventional cytogenetics (CC), interphase FISH and sequential R-banding and FISH analysis as methods for detecting MLL gene rearrangements. 37 acute leukemia patients were studied by CC and interphase FISH. The results showed that among them, 10 cases were 11q23(+)/MLL(+), 2 cases were 11q23(-)/MLL(+) (5.4%), 3 cases were 111q23(+)/MLL(-) (8.1%) and 22 cases were 11q23(-)/MLL(-). For some patients, different results were obtained by using CC and interphase FISH for detecting 11q23/MLL gene rearrangements. After sequential R-banding and FISH analysis for 6 patients, the chromosome related to MLL gene translocation was seen clearly in karyotypes and FISH image. It is concluded that for accurate diagnosis both CC and FISH are needed for detecting 11q23/MLL gene rearrangements, and evaluation is needed in combination of these two results. When necessary, it needs to do sequential R-banding and FISH or molecular analysis.
