JWA gene in regulating committed differentiation of HL-60 cells induced by ATRA, Ara-C and TPA.
- Author:
Qun SHEN
1
;
Jian-Wei ZHOU
;
Rui-Lan SHENG
;
Guang-Rong ZHU
;
Hai-Xia CAO
;
Hua LU
Author Information
1. Department of Hematology, The Jiangsu TCM Hospital Affiliated to Nanjing TCM University, Nanjing 210029, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
pharmacology;
Blotting, Western;
Cell Differentiation;
drug effects;
Cytarabine;
pharmacology;
HL-60 Cells;
HSP27 Heat-Shock Proteins;
HSP70 Heat-Shock Proteins;
biosynthesis;
genetics;
Heat-Shock Proteins;
biosynthesis;
genetics;
Humans;
Intracellular Signaling Peptides and Proteins;
genetics;
Neoplasm Proteins;
biosynthesis;
genetics;
Proto-Oncogene Proteins c-bcl-2;
biosynthesis;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
Tetradecanoylphorbol Acetate;
pharmacology;
Time Factors;
Tretinoin;
pharmacology
- From:
Journal of Experimental Hematology
2005;13(5):804-808
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to explore the role of gene JWA, a novel retinoic acid responsible and cytoskeleton associate gene, in regulating committed differentiation of HL-60 cell and the molecular mechanism in the course of differentiation and apoptosis of leukemic cells. By using FCM, the changes of CD13, CD14, CD15, CD11b and cell cycles were detected in HL-60 cells treated with ATRA (10(-6) mol/L), Ara-C (10 ng/ml) and TPA (10(-8) mol/L) respectively. The samples were determined by semi-quantitative reverse transcript-polymerase chain reaction (RT-PCR) and Western blot for the expression of JWA, Bcl-2, HSP27 and HSP70 at day 0, 2, 4, 6, 8. The results showed that HL-60 cells committedly differentiated into granulocyte-, monocyte-, macrophage-like cells. As a result, JWA was up-regulated in a time-dependent manner, while Bcl-2 was down- regulated at the same time. In ATRA and TPA group, the change of HSP70 had positive correlation with JWA, and negative correlation with Bcl-2. The expression of HSP27 was not detected. Contrast to the cells from APL patient, the expression of JWA need not be activated by ATRA in advance. In this study, we also exposed HL-60 cells in higher dose of Ara-C (20 ng/ml), and JWA expression underwent opposite trend comparing with in lower dose of Ara-C (10 ng/ml). It is concluded that JWA may play double important roles in regulating ATRA and TPA-induced differentiation and apoptosis in leukemic cells. The JWA expression had a negative correlation between induction and cytotoxic response. The difference of JWA expressions between HL-60 cell and ANLL patient cells would be involved in different leukemia pathogenesis.
