Expression of human ERMAP gene in different cell lines.
- Author:
Ying-Yi HE
1
;
Xiao-Hong ZHANG
;
Tie-Zhen YE
;
Zi-Liang WU
Author Information
1. Department of Pediatrics, The First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China.
- Publication Type:Journal Article
- MeSH:
Blood Group Antigens;
genetics;
Butyrophilins;
Cell Line;
Cell Line, Tumor;
Gene Expression Profiling;
Gene Expression Regulation, Neoplastic;
HL-60 Cells;
Hematologic Neoplasms;
genetics;
pathology;
Humans;
Jurkat Cells;
K562 Cells;
RNA, Messenger;
genetics;
metabolism
- From:
Journal of Experimental Hematology
2005;13(5):819-822
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the pattern of human ERMAP gene expression in different cell lines, 15 cell lines derived from hematopoietic tumor, somatic tumor and normal tissue were chosen and were cultured; cells were harvested after culture for 12, 24, 36, 48, 60 and 72 hours; the expression of the human ERMAP was detected by using fluorescent quantitative PCR. The results showed that human ERMAP gene expression was positive in K562 cell line at interval of 12 and 24 hours, and the expression levels were (5.092 +/- 2.331) x 10(6) cps/microl RNA, (5.328 +/- 3.916) x 10(6) cps/microl RNA, respectively; ERMAP gene expression was also positive in ECV304 cell line at interval of 24 hours, and the expression level was (0.84 +/- 0.12) x 10(6) cps/microl RNA; and its expression was negative in other 13 cell lines. It is concluded that human ERMAP gene expression in ECV304 cell line was found first, and its expression in K562 cell line was further confirmed.
