Biological activity of C II TA anti-sense RNA--a novel approach to inhibition of rejection in transplantation.
- Author:
Rong GUO
1
;
Ping ZOU
;
Xin DU
;
Min ZHAG
Author Information
1. Department of Hematology, Guangdong Provincial People Hospital, Guangzhou 510080, China. gh7311@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Cell Line, Tumor;
Flow Cytometry;
Graft Rejection;
genetics;
prevention & control;
HLA-DP Antigens;
biosynthesis;
genetics;
HLA-DR Antigens;
biosynthesis;
genetics;
Humans;
Nuclear Proteins;
genetics;
RNA, Antisense;
genetics;
RNA, Messenger;
genetics;
metabolism;
Reverse Transcriptase Polymerase Chain Reaction;
Trans-Activators;
genetics;
Transfection
- From:
Journal of Experimental Hematology
2005;13(5):848-851
- CountryChina
- Language:Chinese
-
Abstract:
Allo-cell transplant rejection is associated with class II major histocompatibility complex (MHC II), while its transactivator (namely C II TA) regulates MHC II molecules expression strictly and exclusively. The aim of this study was to investigate the inhibiting effect of C II TA anti-sense RNA on MHC II expression. The cDNA for anti-sense RNA recognizing the 114-523 sequence of C II TA (arC II TA) was obtained from Raji cell by RT-PCR, and then inserted into the pcDNA3.1B plasmid (pcDNA3.1B-arC II TA, pD-arC II TA). Raji cells were transfected stably with pD-arC II TA, classic MHC II antigen (HLA-DR, -DP, -DQ) expression was assayed by flow cytometry (FCM). mRNA abundance of C II TA, invariant chain and classic MHC II were detected by RT-PCR. The results showed that compared with control (sense C II TA), the expression inhibition of HLA-DR, -DP, -DQ on pD-arC II TA positive Raji cell was 65.93%, 54.14%, 68.32% respectively. The mRNA contents of C II TA, invariant chain and classic MHC II also decreased. In conclusion, arC II TA inhibited C II TA and thus the family of MHC II molecules were regulated by it, therefore these results provide a novel approach for the control of graft versus host diseases.
