Cold storage of platelet suspension by adding trehalose.
- Author:
Cheng-Yin HUANG
1
;
Rong-Cai TANG
;
Li CAI
;
Yun CUI
;
Lu LIU
Author Information
1. Department of Transfusion Blood, Jiangsu Province Blood Center, Nanjing 210042, China. Huangchengyin1223@126.com
- Publication Type:Journal Article
- MeSH:
Adenosine Diphosphate;
pharmacology;
Animals;
Blood Platelets;
cytology;
drug effects;
Blood Preservation;
methods;
Cell Survival;
Cryopreservation;
methods;
Cryoprotective Agents;
pharmacology;
Platelet Aggregation;
drug effects;
Platelet Transfusion;
Rabbits;
Trehalose;
pharmacology
- From:
Journal of Experimental Hematology
2005;13(5):904-906
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to explore the trehalose method for storing platelets in cold. (51)Cr-labeling platelet was used to detect the platelet survival. The platelet function in vitro was performed by platelet aggregate analyzer. After treatment with 50 mg/ml trehalose at 37 degrees C for 4 hours, the rabbit platelet concentrates (PC, 2.0 x 10(9)/ml) were stored in 4-8 degrees C refrigeration, the platelet function in vitro and survival of chilled platelets transfused into self-rabbits were observed. The results showed that trehalose could protect the chilled rabbit platelets. After PC stored at 20-24 degrees C and 4-8 degrees C for up to 24 hours, the platelet aggregate in vitro in response to 11.2 micromol/L ADP were (75.3 +/- 9.8)% and (80.5 +/- 12.5)%, the survival of PC stored at 20-24 degrees C and 4-8 degrees C for 24, 48, 72 hours after transfused into self-rabbits were (78.1 +/- 7.9)%, (65.4 +/- 6.7)%, (57.5 +/- 7.2)% and (5.1 +/- 2.5)%, (2.8 +/- 2.0)%, (0.9 +/- 0.8)%, respectively. The PC treated with 50 mg/ml trehalose were remained stable for up to 12 days of refrigerated storage in autologous plasma. The platelet aggregate in vitro in response to 11.2 micromol/L ADP at 12 days after stored in refrigeration was (77.8 +/- 9.5)%, the survival on 24, 48, 72 hours of platelet transfused into self-rabbits were (75.7 +/- 11.0)%, (67.0 +/- 8.5)%, (56.8 +/- 8.0)%, respectively. Compared with control group of storing at 20-24 degrees C for 24 h, P > 0.05. In conclusion, trehalose can protect the chilled blood platelets, prolong the circulation of refrigerated rabbit platelets, and not impair chilled rabbit platelet function.
