Expression of HoxA10 in acute leukemia and its significance.
- Author:
Ying HUANG
1
;
Wei-Jia LI
;
Cai-Xia WEI
;
Zhi ZHOU
;
Bo NIE
Author Information
1. Department of Hematology, The First Affiliated Hospital, Kunming Medicine College, Kunming 650031, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Aged;
Aged, 80 and over;
Biomarkers, Tumor;
genetics;
Disease Progression;
Female;
Gene Expression Regulation, Neoplastic;
Homeodomain Proteins;
genetics;
Humans;
Leukemia, Monocytic, Acute;
genetics;
pathology;
therapy;
Leukemia, Myeloid, Acute;
genetics;
pathology;
therapy;
Leukemia, Myelomonocytic, Acute;
genetics;
pathology;
therapy;
Male;
Middle Aged;
RNA, Messenger;
biosynthesis;
genetics;
Reverse Transcriptase Polymerase Chain Reaction
- From:
Journal of Experimental Hematology
2005;13(6):959-963
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the expression of HoxA(10) mRNA in acute leukemia patients and its significance, HoxA(10) level was detected by reverse transcription polymerase chain reaction (RT-PCR) in 50 patients with acute leukemias, 7 healthy volunteers and 3 patients with ITP (idiopathic thrombocytopenic purpura). The regularity of the expression of HoxA(10) gene in acute leukemia and the relationship between HoxA(10) level and the prognosis of leukemia was explored. The results showed that HoxA(10) was expressed in all types of acute myelogenous leukemia; HoxA(10) message was also observed in acute lymphoblastic leukemia patients and part of control groups. 3 normal donors were found not to express HoxA(10). The level of HoxA(10) mRNA of acute myelogenous leukemia patients was significantly higher than that of acute lymphoblastic leukemia patients and controls (P < 0.01). HoxA(10) gene appeared to be more strongly expressed in AML-M(1) and -M(2) subtypes than in AML-M(4) and -M(5) subtypes, and the gene was notable high expressed in acute promyelocytic leukemia. The number of blast and promyeloid cells in the bone marrow was positive related with the level of HoxA (r = 0.635, P < 0.01). The level of HoxA(10) of 9 non-responsive patients was higher than that of 8 remission patients, but there was no significant difference between them (P = 0.258). HoxA(10) was overexpressed in acute myelogenous leukemia. It is concluded that HoxA(10) is a major transcription factor regulating hematopoiesis and a mark to differentiate lymphoid leukemia and myelogenous leukemia, but not a specific gene of cancer. The level of HoxA(10) is related with load of leukemic cells and curative effect, and can affect occurrence and development of leukemia in combination with many cytokines, HoxA(10) may facilitate the leukemia progression with another cofactors.
