Effects of RhG-CSF on apoptosis of HL-60 cell induced by cytarabine.
- Author:
Hong WANG
1
;
Li-Rong SUN
;
Li LI
Author Information
1. Department of Pediactrics, Affiliated Hospital of Qingdao University, Qingdao 266003 China.
- Publication Type:Journal Article
- MeSH:
Antimetabolites, Antineoplastic;
pharmacology;
Apoptosis;
drug effects;
Cell Survival;
drug effects;
Cytarabine;
pharmacology;
Drug Synergism;
Granulocyte Colony-Stimulating Factor;
pharmacology;
HL-60 Cells;
Humans;
Recombinant Proteins
- From:
Journal of Experimental Hematology
2005;13(6):996-998
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the effects of rhG-CSF on apoptosis of HL-60 cell induced by cytarabine (Ara-C), HL-60 cell were cultured with Ara-C, with or without rhG-CSF, and the changes of HL-60 cells were detected by morphology, including Wright's and acridine orange (AO) and ethidium bromide (EB) staining, and MTT assay. The results showed that comparing with Ara-C group, the apoptosis level of rhG-CSF + Ara-C group was enhanced (P < 0.01), and the OD value of MTT assay declined (P < 0.01). It is concluded that rhG-CSF can induce the apoptosis of HL-60 cells and enhance the apoptosis induced by Ara-C.
