Peripheral stem cell mobilization with medium dose of G-CSF in normal donors.
- Author:
Su-Xia LI
1
;
Wang-Ming DA
;
Chun-Ji GAO
;
Wen-Rong HUANG
;
Hai-Jie JIN
Author Information
1. Department of Hematology, General Hospital of PLA, Beijing 100853, China.
- Publication Type:Journal Article
- MeSH:
Adolescent;
Adult;
Antigens, CD34;
analysis;
Blood Donors;
Female;
Flow Cytometry;
Graft vs Host Disease;
prevention & control;
Granulocyte Colony-Stimulating Factor;
administration & dosage;
Hematopoietic Stem Cell Mobilization;
methods;
Humans;
Male;
Middle Aged;
Peripheral Blood Stem Cell Transplantation;
methods;
Recombinant Proteins;
T-Lymphocyte Subsets;
cytology;
drug effects;
immunology;
Time Factors
- From:
Journal of Experimental Hematology
2005;13(6):1038-1040
- CountryChina
- Language:Chinese
-
Abstract:
The study was aimed to investigate the mobilization effect of medium dose of granulocyte colony stimulating factor (G-CSF) in allogeneic peripheral stem cell transplantation and changes of T lymphocyte subgroup in PBMNC before and after mobilization. G-CSF was administered at 600 microg/d (i.e. 300 microg i.v. twice a day) for successive 5 days to 31 matched sibling or unrelated donors for the mobilization. Stem cells were harvested on the fourth day. FACS was used to analyze the NC, MNC and T lymphocyte subgroups. The results showed that the number of NC, MNC, CD34(+) cells and CFU-GM in dose of 600microg/d significantly increased (P < 0.05), compared with 300 microg/d; the time for hematological reconstruction was significantly shortened (P < 0.05); the ratio of adverse effects was not obviously increased (P > 0.05) and the median percentage of CD3(+) lymphocytes before mobilization was 46.96% [(32.36-57.45)%], but 40.94% [(25.31-48.9)%] after mobilization, while the ratio of CD4(+)/CD8(+) did not significantly changed. It is concluded that the administration of G-CSF 600 microg/d in allo-PBSCT has a good effect in the mobilization of PBSC with minor side effects, which can markedly promote hematopoietic reconstitution after transplantation. The relative amount of CD3(+) lymphocytes significantly decreased and the ratio of CD4(+)/CD8(+) remained unchanged, which may lead to alleviation of a GVHD after PBSCT.
