Effect of GM-CSF on expansion and differentiation of CD34+ megakaryocyte progenitor cells from cord blood in vitro.
- Author:
Shu CHEN
1
;
Fa-Ming ZHU
;
Ji HE
;
Jin-Hui LIU
;
Fei QIN
;
Li-Xing YAN
Author Information
1. Institute of Blood Transfusion, Blood Center of Zhejiang Province, Hangzhou 310006, China.
- Publication Type:Journal Article
- MeSH:
Adult;
Antigens, CD34;
analysis;
Cell Differentiation;
drug effects;
Cell Proliferation;
drug effects;
Cells, Cultured;
Dose-Response Relationship, Drug;
Female;
Fetal Blood;
cytology;
immunology;
Granulocyte-Macrophage Colony-Stimulating Factor;
pharmacology;
Hematopoietic Stem Cells;
cytology;
drug effects;
immunology;
Humans;
Megakaryocytes;
cytology;
drug effects;
immunology
- From:
Journal of Experimental Hematology
2005;13(6):1041-1043
- CountryChina
- Language:Chinese
-
Abstract:
To study the effect of GM-CSF on in vitro expansion of megakaryocyte progenitor cells from cord blood, CD34(+) cells isolated by magnetic cell sorting system (MACS) were cultured in serum-free medium containing TPO, IL-3, SCF and with or without various concentrations of GM-CSF (5, 20, 100 ng/ml). The numbers of MNC, proportion of CD34(+)CD41(+) cells and CFU-MK were measured at 6, 10 and 14 days. The results showed that the expansion of MNC and proportion of CD41(+) cells was accelerated distinctly by various concentrations of GM-CSF after 14 days, while 20 and 100 ng/ml GM-CSF exhibited higher expansion effect than that of 5 ng/ml. TPO + IL-3 + SCF with 5 ng/ml or 20 ng/ml GM-CSF could stimulate the formation of CFU-MK, while TPO + IL-3 + SCF with 100 ng/ml GM-CSF could inhibit it. It is concluded that GM-CSF can accelerate the expansion of megakaryocyte progenitor cells from CD34(+) cells in cord blood in the serum-free medium containing TPO + IL-3 + SCF.
