Influence of hepatocyte growth factor on biological characteristics of bone marrow-derived mesenchymal stem cells.
- Author:
Hong-Jun LIU
1
;
Hai-Feng DUAN
;
Zhuo-Zhuang LU
;
Hua WANG
;
Qun-Wei ZHANG
;
Zu-Ze WU
;
Li-Sheng WANG
Author Information
1. Department of Experimental Hematology, Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Anoikis;
drug effects;
Bone Marrow Cells;
cytology;
drug effects;
metabolism;
Cell Movement;
drug effects;
Cell Proliferation;
drug effects;
Cells, Cultured;
Hepatocyte Growth Factor;
pharmacology;
Humans;
Immunohistochemistry;
Mesenchymal Stromal Cells;
cytology;
drug effects;
metabolism;
Proto-Oncogene Proteins c-met;
biosynthesis
- From:
Journal of Experimental Hematology
2005;13(6):1044-1048
- CountryChina
- Language:Chinese
-
Abstract:
Hepatocyte growth factor (HGF) is one of major growth factors in the bone marrow microenvironments with which the proliferation, differentiation and migration of bone marrow-derived mesenchymal stem cells were closely contacted. However, its roles in the regulation of proliferation, differentiation and migration of bone marrow-derived mesenchymal stem cells remain unclear. This study was aimed to investigate the effect of HGF on biological characteristics of bone marrow-derived mesenchymal stem cells. Expression of c-Met, the receptor for HGF was detected by immunohistochemistry assay, cell proliferation was determined by MTT, activity of ALP was quantitatively assayed, cell migration and anoikis-induced MSC apoptosis were analyzed. The results showed that HGF not influenced the proliferation and osteogenic differentiation of bone marrow-derived mesenchymal stem cells. Treatment of bone marrow-derived mesenchymal stem cells with recombinant human hepatocyte growth factor resulted in inhibition of anoikis-induced apoptosis. HGF significantly stimulated the migration of bone marrow-derived mesenchymal stem cells. Both PI-3 kinase and MAPK kinase were proved to be involved in HGF-induced migration. It is concluded that HGF/c-Met signal regulates the apoptosis and migration of bone marrow-derived mesenchymal stem cells.
