A method for Rhesus box test.
- Author:
Jiong-Cai LAN
1
;
Hua-You ZHOU
;
Rong XIA
;
Qiong CAO
;
Yan-Chao XING
;
Gui-Zhi PANG
;
Can WU
;
Quan-Ke YANG
Author Information
1. Department of Blood Transfusion, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. gzlanjc@tom.com
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Haplotypes;
Heterozygote;
Homozygote;
Humans;
Polymerase Chain Reaction;
methods;
Rh-Hr Blood-Group System;
analysis;
genetics
- From:
Journal of Experimental Hematology
2005;13(6):1103-1105
- CountryChina
- Language:Chinese
-
Abstract:
To study the method for Rhesus box test and its significance, the sequence specific primers of upstream, downstream and hybrid Rhesus boxes were designed according to RhD gene sequence; the upstream, downstream and hybrid Rhesus boxes were determined by PCP-SSP and mismatched PCR. The results showed that this method was confirmed by DNA Standard test. It was shown that in unrelative RhD positive individuals RHD(+)/RHD(-), RHD(+)/RHD(+) genotype accounted for 9.00%, 91.00% respectively, and in RhD negative individuals RHD(+)/RHD(-), RHD(+)/RHD(+), RHD(-)/RHD(-) genotype were 26.14%, 3.92%, 69.94% respectively. It is concluded that the method of Rhesus box test was confirmed to be reliable and can be used for the identification of RhD haplotype gene structure, as well as for study on inheritance, clinical transfusion and neonatal hemolytic diseases.
