Polymorphism of killer cell immunoglobulin-like receptors gene family in Zhejiang Han population.
- Author:
Fa-Ming ZHU
1
;
Kan JIANG
;
Qin-Feng LÜ
;
Wei ZHANG
;
Hai-Qin ZHANG
;
Qi-Hua FU
;
Li-Xing YAN
Author Information
1. Blood Center of Zhejiang Province, Hangzhou 310006, China.
- Publication Type:Journal Article
- MeSH:
China;
Gene Frequency;
Genotype;
Haplotypes;
Humans;
Polymerase Chain Reaction;
methods;
Polymorphism, Genetic;
Receptors, KIR;
genetics
- From:
Journal of Experimental Hematology
2005;13(6):1109-1112
- CountryChina
- Language:Chinese
-
Abstract:
To analyze killer immunoglobulin (Ig)-like receptor (KIR) gene content and allelic polymorphism in Zhejiang Han population, samples were genotyped by polymerase chain reaction sequence-specific primers (PCR-SSP). The results demonstrated that all 17 KIR genes could be observed in the population. All individuals contained 2DL4, 3DL2 and 3DL3 genes. The frequencies of these genes was 1.00. 2DL1, 2DL3, 2DP1, 3DP1*003, 3DL1, 2DS4*001/002 loci were more common, their frequencies were 0.902, 0.902, 0.902, 0.902, 0.7598, 0.5615 respectively, while the frequencies of 2DL2, 2DL5A, 2DL5B, 2DS1, 2DS2, 2DS3, 2DS4*003, 2DS5, 3DS1 and 3DP1*001/002 were relatively lower. The A KIR haplotype was the most prevalent (74.7%) in Zhejiang Han population and there were 12 different KIR haplotypes in total, the most common was 2 (53.0%). Twenty six different genotypes have been found in the population, AJ (2, 2) and AF (1, 2) showed higher frequencies, followed by AH (2, 5), NN2 (2, 6), AI (1, 5) and AG (1, 1). Fifteen of these genotypes have not been found in Caucasians so far and four new KIR profiles could not be assigned to the haplotypes according to standard assign method. In conclusion, there are distinctive frequencies of KIR gene content, haplotype as well as genotype in Zhejiang Han population.
