Enhancement of herpes simplex virus-1 glycoprotein-D DNA vaccine induced specific immune responses by coimmunization with interleukin-2 genetic adjuvant.
- Author:
Xiao-juan LIU
1
;
Ming-zhao ZHU
;
Guo-xing SONG
;
Yu-fei XU
;
Hong-wei LIU
;
Sheng WANG
;
Bao-ling YANG
;
Fang-tian DONG
;
Xue-mei XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Viral; blood; COS Cells; Cell Proliferation; Cercopithecus aethiops; DNA; genetics; Female; Herpesvirus 1, Human; pathogenicity; Hypersensitivity, Delayed; immunology; Immunization; Immunoglobulin G; blood; Interferon-gamma; blood; Interleukin-2; biosynthesis; genetics; Mice; Mice, Inbred BALB C; Random Allocation; Th1 Cells; cytology; Transfection; Vaccines, DNA; immunology; Viral Envelope Proteins; biosynthesis; genetics; Viral Vaccines; immunology
- From: Acta Academiae Medicinae Sinicae 2005;27(1):67-72
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the immune responses and protection from virus challenge, induced by the coinjection of IL-2cDNA with herpes simplex virus type 1 (HSV-1) glycoprotein-D (gD) DNA vaccine.
METHODSTwo DNA vaccines (pgD and pIL-2) were constructed by inserting the gD gene and IL-2 cDNA into the eukaryotic expression vector pcDNA3.1, respectively. The BALB/c mice were inoculated intramuscularly three times at 2-week intervals. Two weeks after the final immunization, mice were bled for antibody assay and spleen cells were separated for Th cell proliferation and cytokine assays. Delayed type hypersensitivity (DTH) response was detected by the pinna-swelling test. Corneal protection under HSV-1 virus challenge was continuously observed with slit-lamp microscope.
RESULTSIL-2 cDNA coinjection remarkably enhanced the specific IgG2a level when compared with gD plasmid vaccination alone. Th cell proliferation and secretion of cytokines (IL-2 and IFN-gamma) were significantly increased by IL-2 cDNA coinjection. However, the production of IL-10 was inhibited. The DTH response was also enhanced by IL-2 coinjection. When the mice were challenged with HSV-1, the cornea epithelial lesions were significantly alleviated by IL-2 coinjection as compared with gD vaccination alone.
CONCLUSIONIL-2 cDNA can enhance both the humoral and cellular immune responses, and thus increase the vaccine potency.