Rat alveolar type II injured by bleomycin.
- Author:
Lu KONG
1
;
Zhi-gang WANG
;
Jian-zhao NIU
;
Ji-feng WANG
;
Huan JIN
;
Mei-juan YANG
;
Ling-qiao WANG
;
Bing-hua TANG
;
Qiu-ju ZHANG
;
Heng-jing TU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Aquaporin 1; biosynthesis; genetics; Bleomycin; administration & dosage; toxicity; Cells, Cultured; Dose-Response Relationship, Drug; Epithelial Cells; drug effects; metabolism; Hypoxia; chemically induced; metabolism; pathology; Male; Pulmonary Alveoli; cytology; drug effects; Pulmonary Surfactant-Associated Protein A; biosynthesis; genetics; Pulmonary Surfactant-Associated Protein B; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; Random Allocation; Rats; Rats, Sprague-Dawley; Time Factors
- From: Acta Academiae Medicinae Sinicae 2005;27(1):81-86
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore dysfunction mechanism of rat alveolar type II (AT-II) injured by bleomycin (BLM).
METHODSSD rats were injected with a single intratracheal dose of bleomycin or control saline. On day 7, 14, and 28 following intratracheal bleomycin or saline instillation, animals were killed under overdose of 1.5% sodium pentobarbital (0.25 ml/100 g, i.p.) and bronchoalveolar lavage fluid (BALF) from the lung was tested for the activity of pulmonary surfactant (PS) by the Whihelmy Film Balance. Several concentrations of bleomycin stimulated the culture of rat AT-II cells, and surfactant protein (SP) A, B, and aquaporin-1 (AQP) mRNA were analyzed by fluorescent quantitative polymerase chain reaction (FQ-PCR).
RESULTSThe activity of PS and hypoxemia significantly decreased on day 7 and improved on day 14 and completely recovered to normal status on day 28. SP-A, B, and AQP-1 mRNA expression in BLM-stimulated group were significantly lower than those in the control group (P<0.001).
CONCLUSIONBLM-injured AT-II cells decrease the levels of SP-A, B, and AQP-1 mRNA and cause PS dysfunction, resulting in hypoxemia and pneumonedema.
