Altered expression of the HSD17B4 gene in esophageal squamous cell carcinoma and loss of heterozygosity analysis.
- Author:
Xiao-dong LI
1
;
Xiang-yang LIU
;
Xiao-ping HUANG
;
Jian-hua FU
;
Yi HU
;
Xin XU
;
Yan CAI
;
Ya-ling HAN
;
Tie-hua RONG
;
Min WU
;
Qi-min ZHAN
;
Ming-rong WANG
Author Information
- Publication Type:Journal Article
- MeSH: 17-Hydroxysteroid Dehydrogenases; biosynthesis; genetics; Adult; Aged; Carcinoma, Squamous Cell; genetics; Down-Regulation; Enoyl-CoA Hydratase; biosynthesis; genetics; Esophageal Neoplasms; genetics; Female; Gene Expression; Gene Expression Regulation, Neoplastic; genetics; Genes, Tumor Suppressor; Humans; Hydro-Lyases; Loss of Heterozygosity; Male; Microsatellite Repeats; Middle Aged; Multienzyme Complexes; biosynthesis; genetics; Peroxisomal Multifunctional Protein-2; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: Acta Academiae Medicinae Sinicae 2005;27(3):270-273
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the alteration of the gene HSD17B4 in esophageal squamous cell carcinoma and its potential significance.
METHODSThe mRNA expression and loss of heterozygosity (LOH) of HSD17B4 in 40 primary esophageal tumors were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and microsatellite analysis with the intragenic marker D5S1384 of the gene.
RESULTSThe frequencies of allelic loss of D5S1384 and the rate of down-regulation of gene HSD17B4 were 46.2% and 62.5%, respectively.
CONCLUSIONHSD17B4 may be a candidate tumor suppressor gene associated with esophageal squamous cell carcinoma.
