Nuclear localization region in soluble adenylyl cyclase.
- Author:
Qi-ping FENG
1
;
Jin ZUO
;
Yan MENG
;
Fu-de FANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenylyl Cyclases; genetics; Diabetes Mellitus, Type 2; genetics; Female; Genetic Predisposition to Disease; genetics; Genetic Testing; Humans; Male; Microscopy, Confocal; Nuclear Proteins; genetics; Polymorphism, Single Nucleotide; genetics
- From: Acta Academiae Medicinae Sinicae 2005;27(3):280-284
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo locate the region responsible for nuclear localization of protein sAC.
METHODSThe eukaryotic expression vector of vairous sAC deletion mutants were transfected into Hela cells. The localization of each mutant was observed using confocal microscope.
RESULTSFor some mutants, the localization of sAC changed. Deletion of some region made it unable to locate in the nuclear.
CONCLUSIONIt is possible to figure out that the nucleotide region (739-1038 and 1045-1261) take charge of nuclear localization of sAC.
