Recombinant human pigment epithelium-derived factor inhibits the proliferation of the endothelial cells from blood vessels.
- Author:
Bin YIN
1
;
Fei-chan QIU
;
Zhong-wei WEN
;
Sheng-tao ZHU
;
Jian-gang YUAN
;
Bo-qin QIANG
;
Xiao-zhong PENG
Author Information
- Publication Type:Journal Article
- MeSH: Angiogenesis Inhibitors; pharmacology; Apoptosis; drug effects; Base Sequence; Cell Division; drug effects; Cells, Cultured; Cloning, Molecular; Endothelium, Vascular; cytology; Eye Proteins; pharmacology; Humans; Molecular Sequence Data; Nerve Growth Factors; pharmacology; Prokaryotic Cells; metabolism; Recombinant Proteins; pharmacology; Serpins; pharmacology; Umbilical Veins; cytology
- From: Acta Academiae Medicinae Sinicae 2005;27(3):295-299
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo express and purify the recombinant human pigment epithelium-derived factor (PEDF) which inhibits the proliferation of the endothelium cells from blood vessel in E.coli.
METHODSPEDF gene was inserted into the prokaryotic expression vector pGEX-4T-2. The recombinant protein PEDF was expressed in E.coli BL-21, and purified by the GST Sepharose 4B affinity column. The recombinant human PEDF protein was identified by Western blot and mass spectrum. The biological activity of the recombinant human PEDF protein was measured by using MTT.
RESULTSThe 46 kDa recombinant human PEDF protein was obtained. It significantly inhibited the proliferation of the human umbilical vein cell line HUVEC.
CONCLUSIONThe recombinant human PEDF with anti-angiogenesis activity protein may be successfully purify through prokaryotic expression.
