Inhibition of multidrug resistance related P-gp expression in human neuroblastoma by antisense peptide nucleic acid.
- Author:
Hua GUO
1
;
Qing-lin ZHANG
;
Jian ZHANG
;
Cheng-wei WANG
;
Jian-xin KONG
;
Fu-sheng LIU
;
Dao-xin MA
;
Ji-feng BIAN
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; biosynthesis; genetics; Animals; Drug Resistance, Multiple; genetics; Humans; Nervous System Neoplasms; metabolism; Neuroblastoma; metabolism; Oligonucleotides, Antisense; pharmacology; Peptide Nucleic Acids; pharmacology; RNA, Messenger; biosynthesis; genetics; Transfection; Tumor Cells, Cultured
- From: Acta Academiae Medicinae Sinicae 2005;27(3):300-304
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the efficiency of a peptide nucleic acid (PNA) delivery system by using liposome via PNA-DNA hybrids and to test the inhibitive action of antisense PNA on expression of multidrug resistance (MDR) related P-glycoprotein (P-gp) in human neuroblastoma cell line SK-N-SH.
METHODSTwo antisense PNAs were designed targeting at MDR-1 mRNA and then combined with partially complement DNAs respectively. The hybrids were delivered into cells using cationic liposome. The transfection efficiency, expression of P-gp and MDR-1 mRNA, intracellular adarimycin (ADM) were measured by flow cytometry, reverse transcription-polymerase chain reaction (RT-PCR), and high performance liquid chromatography (HPLC).
RESULTSTransfection of PNA increased the cell average fluorescence intensity significantly and the extent of increase was dependent on the concentration of PNA. After being transfected by both PNAs, P-gp expression of SK-N-SH cells decreased significantly and the intracellular ADM level was increased by about 3 times. The level of MDR-1 mRNA expression slightly decreased after transfection, but no statistical significance was observed.
CONCLUSIONSPNA can be delivered into tumor cells in form of PNA-DNA hybrids by cationic liposome. Properly designed antisense PNA can inhibit MDR related P-gp expression of SK-N-SH cells efficiently and specifically.
